%0 Journal Article
%4 sid.inpe.br/plutao/2016/06.13.21.00
%2 sid.inpe.br/plutao/2016/06.13.21.00.16
%@doi 10.3390/ijms17050672
%@issn 1422-0067
%F lattes: 4809959472520732 2 ZimbardiCCAMRAJF:2016:PoApDy
%T A high redox potential laccase from pycnoporus sanguineus RP15: potential application for dye decolorization
%D 2016
%A Zimbardi, Ana,
%A Camargo, Priscila de Fátima,
%A Carli, Sibeli,
%A Aquino Neto, Sidney,
%A Meleiro, Luana P.,
%A Rosa, José C.,
%A Andrade, Adalgisa R. de,
%A Jorge, João A.,
%A Furriel, Rosa P. M.,
%@affiliation Universidade de São Paulo (USP)
%@affiliation Instituto Nacional de Pesquisas Espaciais (INPE)
%@affiliation Universidade de São Paulo (USP)
%@affiliation Universidade de São Paulo (USP)
%@affiliation Universidade de São Paulo (USP)
%@affiliation Universidade de São Paulo (USP)
%@affiliation Universidade de São Paulo (USP)
%@affiliation Universidade de São Paulo (USP)
%@affiliation Universidade de São Paulo (USP)
%@electronicmailaddress luccilatorre@usp.br
%@electronicmailaddress priscila@lcp.inpe.br
%@electronicmailaddress sibelicarli@usp.br
%@electronicmailaddress netaoaquino@gmail.com
%@electronicmailaddress luanapm@usp.br
%@electronicmailaddress jcrosa@fmrp.usp.br
%@electronicmailaddress ardandra@ffclrp.usp.br
%@electronicmailaddress joajorge@ffclrp.usp.br
%B International Journal of Molecular Sciences
%V 17
%N 5
%P 672
%K Pycnoporus sanguineus, solid state fermentation, response surface methodology, laccase, purification, characterization, decolorization, remazol brilliant blue R, Reactive blue 4, bromophenol blue.
%X Laccase production by Pycnoporus sanguineus RP15 grown in wheat bran and corncob under solid-state fermentation was optimized by response surface methodology using a Central Composite Rotational Design. A laccase (Lacps1) was purified and characterized and the potential of the pure Lacps1 and the crude culture extract for synthetic dye decolorization was evaluated. At optimal conditions (eight days, 26 ˝C, 18% (w/w) milled corncob, 0.8% (w/w) NH4Cl and 50 mmol¨ L ´1 CuSO4, initial moisture 4.1 mL¨ g ´1 ), the laccase activity reached 138.6 ˘ 13.2 U¨ g ´1 . Lacps1 was a monomeric glycoprotein (67 kDa, 24% carbohydrate). Optimum pH and temperature for the oxidation of 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) were 4.4 and 74.4 ˝C, respectively. Lacps1 was stable at pH 3.08.0, and after two hours at 5560 ˝C, presenting high redox potential (0.747 V vs. NHE). ABTS was oxidized with an apparent affinity constant of 147.0 ˘ 6.4 µmol¨ L ´1 , maximum velocity of 413.4 ˘ 21.2 U¨ mg´1 and catalytic efficiency of 3140.1 ˘ 149.6 L¨ mmol´1 ¨ s ´1 . The maximum decolorization percentages of bromophenol blue (BPB), remazol brilliant blue R and reactive blue 4 (RB4), at 25 or 40 ˝C without redox mediators, reached 90%, 80% and 60%, respectively, using either pure Lacps1 or the crude extract. This is the first study of the decolorization of BPB and RB4 by a P. sanguineus laccase. The data suggested good potential for treatment of industrial dye-containing effluents.
%@language en
%3 zimbardi_high.pdf